BP005363: Biotinylated Human CD40/TNFRSF5 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human CD40/TNFRSF5 Protein, (amino acids Glu21-Arg193) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human CD40 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD40 Ligand (Trimer) protein with the C-hFc tag with the EC50 of 9.5 ng/ml determined by ELISA (QC test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005700: Cynomolgus CD40/TNFRSF5 Protein with the C-His Tag
Source: The recombinant Cynomolgus CD40/TNFRSF5 Protein, (amino acids Glu21-Arg193) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus CD40 protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD40 Ligand (Trimer) protein with the C-hFc tag with the EC50 of 12.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005370: Human CD40/TNFRSF5 Protein with the C-His Tag
Source: The recombinant Human CD40/TNFRSF5 Protein, (amino acids Glu21-Arg193) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD40 protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD40 Ligand (Trimer) protein with the C-hFc tag with the EC50 of 79.7 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005290: Human CD40/TNFRSF5 Protein with the C-hFc Tag
Source: The recombinant Human CD40/TNFRSF5 Protein, (amino acids Glu21-Arg193) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD40 Ligand Trimer protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD40 protein with the C-hFc tag with the EC50 of 0.7 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005360: Mouse CD40/TNFRSF5 Protein with the C-His Tag
Source: The recombinant Mouse CD40/TNFRSF5 Protein, (amino acids Val24-Arg193) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse CD40 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse CD40 Ligand Trimer protein with the C-hFc tag with the EC50 of 25.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.